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A Robust RP-HPLC Analytical Method Development and Validation of Cilnidipine and Olmesartan in Antihypertensive Formulations

Lokesh Gurav*, Sandip Ravandale, Harshali Jadhav, A V Patil

This study presents a validated RP-HPLC method for the simultaneous determination of Cilnidipine and Olmesartan in pharmaceutical formulations. The mobile phase consisted of a mixture of methanol and buffer, with variations in composition. The retention times for Cilnidipine and Olmesartan were found to be 4.89 minutes and 7.48 minutes, respectively. The method exhibited excellent linearity with correlation coefficients (R²) above 0.999 for Cilnidipine and above 0.9994 for Olmesartan. Good precision was observed with % RSD values below 1.3% for both intra-day and inter-day analysis. The recovery study demonstrated accurate and reliable quantification of the drugs, with recovery percentages ranging from 98.5% to 101.4%. The method also exhibited robustness, as minor variations in pH, mobile phase composition and flow rate did not significantly affect the results. Furthermore, the method showed high sensitivity with low LOD and LOQ values of 0.21 µg and 0.39 µg for Cilnidipine, and 0.63 µg and 1.18 µg for Olmesartan, respectively. Overall, the developed HPLC method provides a reliable and sensitive approach for the analysis of Cilnidipine and Olmesartan in pharmaceutical formulations.